Identify Key Sequence Features to Improve CRISPR sgRNA Efficacy
نویسندگان
چکیده
منابع مشابه
Sequence determinants of improved CRISPR sgRNA design.
The CRISPR/Cas9 system has revolutionized mammalian somatic cell genetics. Genome-wide functional screens using CRISPR/Cas9-mediated knockout or dCas9 fusion-mediated inhibition/activation (CRISPRi/a) are powerful techniques for discovering phenotype-associated gene function. We systematically assessed the DNA sequence features that contribute to single guide RNA (sgRNA) efficiency in CRISPR-ba...
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ژورنال
عنوان ژورنال: IEEE Access
سال: 2017
ISSN: 2169-3536
DOI: 10.1109/access.2017.2775703